Part:BBa_K3973000:Design
UPC2 transcriptional regulator
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 664
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 204
Illegal AgeI site found at 1120 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The 2021 Hopkins iGEM team predicted that upc2, when expressed recombinantly within bacteria, can be used as a transcriptional repressor depending on the position of an SRE. If SREs are placed following the polymerase binding site, upc2 can block the production of mRNA transcripts and thus functions similarly to the esabox/esaR system.
Source
UPC2 is native to several fungal species including Candida albicans. The endogenous function of upc2 is as a transcriptional activator, inducing the expression of ergosterol production genes by binding to an SRE upstream of the polymerase binding site and encouraging the assembly of transcription factors in response to ergosterol depletion. Ergosterol binding to upc2 in the cytoplasm obscures the nuclear localization and DNA binding domains of the upc2 dimer and thus prevents it from inducing the expression of ergosterol production genes.
References
Davies, B. S., Wang, H. S., & Rine, J. (2005). Dual activators of the sterol biosynthetic pathway of saccharomyces cerevisiae: Similar activation/regulatory domains but different response mechanisms. Molecular and Cellular Biology, 25(16), 7375–7385. https://doi.org/10.1128/mcb.25.16.7375-7385.2005
Vik, Å., & Rine, J. (2001). UPC2P and Ecm22p, dual regulators of sterol biosynthesis in saccharomyces cerevisiae. Molecular and Cellular Biology, 21(19), 6395–6405. https://doi.org/10.1128/mcb.21.19.6395-6405.2001
Yang, H., Tong, J., Lee, C. W., Ha, S., Eom, S. H., & Im, Y. J. (2015). Structural mechanism of ergosterol regulation by fungal sterol transcription factor UPC2. Nature Communications, 6(1). https://doi.org/10.1038/ncomms7129